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Following, the ratio of Ex4a(+)WT1 isoform to 17AA(+) WT1 isoform was determined by RT-PCR employing Ex4-forward and Ex6-reverse primer pair in WT1-expressing cancer cell strains (LU99B and K562) and decided to get around 1/2 and 1/4 in LU99B and K562 most cancers cells, respectively (Fig 4D). These effects indicated that the Ex4a(+)WT1 isoform was expressed being a minor isoform together with the

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